Functional reconstitution of a chloride channel bares its soul
نویسندگان
چکیده
منابع مشابه
Functional reconstitution of a chloride channel bares its soul.
Chloride channels have been called the poor cousins of the aristocratic cation channels because their physiological functions involve “cleaning up the mess after the party” (1). Although Cl channels are not as glamorous as the Na, Ca, and K channels dominating neuronal signaling, their functions are indispensable (2), as exemplified by cholera caused by superactivation of a Cl channel (CFTR) in...
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15 صفحه اولFunctional Reconstitution of a Prokaryotic K 1 Channel
SliK, a K 1 channel encoded by the Streptomyces KcsA gene, was expressed, purified, and reconstituted in liposomes. A concentrative 86 Rb 1 flux assay was used to assess the ion transport properties of SliK. SliK-mediated ionic flux shows strong selectivity for K 1 over Na 1 and is inhibited by micromolar concentrations of Ba 2 1 , mirroring the basic permeation characteristic of eukaryotic K 1...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 2013
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.1319415110